DEVELOPMENT AND VALIDATION OF AN RP-HPLC METHOD FOR DEFERIPRONE ESTIMATION IN PHARMACEUTICAL DOSAGE FORM

Objectives: For the simultaneous estimation of deferiprone in pharmaceutical formulations, a high-performance liquid chromatographic technique that is straightforward, reproducible, and effective was developed. Methods: The Advanced Technologies Ltd HPLC system with UV-2075 UV-Vis detector, P-2080 pump, Hypersil C18 column (250 × 4.6 mm) used to perform separation. ACN water make up mobile phase (55:45 v/v). solvent flowing at rate 1.0 mL/min. 4.7 g sodium dihydrogen orthophosphate 1 mL triethyl amine were as buffer, orthophosphoric acid bring pH solution 4.0 ± 0.05. sample volume 20 μL, well temperature range, kept room temperature. Results: method approved for rapid screening confirmed conformance ICH guidelines. purpose determining presence active ingredients, an RP-HPLC analysis model Uv spectrophotometer has been drug determination retention time (4.960 min), which crucial routine analysis. method’s high sensitivity indicated by low identification qualifying limits. Conclusion: developed simultaneously Deferiprone formulations found be more accurate, precise, selective.